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Overall study design regarding the association between circulating inflammatory proteins and Fuchs endothelial corneal dystrophy.

Journal: Translational Vision Science & Technology

Article Title: Circulating Inflammatory Proteins and Fuchs Endothelial Corneal Dystrophy: A Mendelian Randomization and Bioinformatics-Based Investigation

doi: 10.1167/tvst.15.5.6

Figure Lengend Snippet: Overall study design regarding the association between circulating inflammatory proteins and Fuchs endothelial corneal dystrophy.

Article Snippet: Immortalized human corneal endothelial cells (CECs, B4G12) were acquired from Creative Bioarray (Shirley, NC, USA).

Techniques:

TNFRSF11B is highly expressed in FECD-affected tissues and H 2 O 2 stimulated immortalized human corneal endothelial cells. ( A – E ) Shown the differential expression of TNFRSF11B in four GEO datasets. ( F ) Immortalized human corneal endothelial cells were stimulated with 200 µM H 2 O 2 for 24 hours, and the mRNA expression of TNFRSF11B was examined by RT-qPCR. *** P < 0.001.

Journal: Translational Vision Science & Technology

Article Title: Circulating Inflammatory Proteins and Fuchs Endothelial Corneal Dystrophy: A Mendelian Randomization and Bioinformatics-Based Investigation

doi: 10.1167/tvst.15.5.6

Figure Lengend Snippet: TNFRSF11B is highly expressed in FECD-affected tissues and H 2 O 2 stimulated immortalized human corneal endothelial cells. ( A – E ) Shown the differential expression of TNFRSF11B in four GEO datasets. ( F ) Immortalized human corneal endothelial cells were stimulated with 200 µM H 2 O 2 for 24 hours, and the mRNA expression of TNFRSF11B was examined by RT-qPCR. *** P < 0.001.

Article Snippet: Immortalized human corneal endothelial cells (CECs, B4G12) were acquired from Creative Bioarray (Shirley, NC, USA).

Techniques: Quantitative Proteomics, Expressing, Quantitative RT-PCR